Do these bags connect directly to the Medela freestyle pump, or do I have to pump into a bottle first?

I connect them to my Medela pump. They sell an adaptor for the Medela to pump into their bags. Medela bags are smaller and I always pump at least 4 oz from one of my breasts. So I just cut a slit in the flap of these bags (There is a double purple line that I use) and use the Medela adaptor part. Works great! best tip I got as a BF/working/pumping mom. I hated coming home after 14 hours and having to transfer milk and wash and sterilize bottles.

Can I reuse these bags or must throw away after use?

These bags are single use and are supposed to be thrown away. There's no way to "re-sanitize" them, so to speak.

Can I pump directly into these bags from a Ameda pump?

I do it that way. i use tape to attach the tops of the bags onto the pump. i pump both sides at the same time.The 4 reasons i pump directly into the bag is 1-less waste, i dont waste/spill a single drop transferring from bottle to bag2-less work bc no bottle to wash out3-saves time bc i dont have to pour from bottle to bag or wash out the bottle4-less bacterial contamination because there's no transferring step

Brand	Lansinoh
Model Number	LA-008
Product Dimensions	8.89 x 8.89 x 15.88 cm; 771.11 g
Material	Plastic
Quantity	200
Laser Class	Lightweight
Included Components	Breast Milk Storage Bag
Are Batteries Included	No
Item Weight	771 g

Lansinoh ランシノ母乳保存袋 100 袋 並行輸入品

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Lansinoh　ランシノ母乳保存袋 100 袋並行輸入品